HDMX Folds the Nascent p53 mRNA following Activation by the ATM Kinase
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Regulated protein synthesis via changes in mRNA structures forms an important part of how prokaryotic cells adapt protein expression in response to changes in the environment. Little is known regarding how this concept has adapted to regulate mRNA translation via signaling pathways in mammalian cells. Here, we show that following phosphorylation by the ataxia telangiectasia mutated (ATM) kinase at serine 403, the C-terminal RING domain of HDMX binds the nascent p53 mRNA to promote a conformation that supports the p53 mRNA-HDM2 interaction and the induction of p53 synthesis. HDMX and its homolog HDM2 bind the same p53 internal ribosome entry sequences (IRES) structure but with different specificity and function. The results show how HDMX and HDM2 act as nonredundant IRES trans-acting factors (ITAFs) to bring a positive synergistic effect on p53 expression during genotoxic stress by first altering the structure of the newly synthesized p53 mRNA followed by stimulation of translation. © 2014 Elsevier Inc.
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ATM protein; messenger RNA; protein; protein HDMX; protein p53; serine; serine 403; trans acting factor; unclassified drug; article; conformation; controlled study; enzyme phosphorylation; human; human cell; protein synthesis; ribosome; Animals; Ataxia Telangiectasia Mutated Proteins; Base Sequence; DNA Damage; Gene Expression Regulation; Humans; Inverted Repeat Sequences; Molecular Sequence Data; Nuclear Proteins; Phosphorylation; Protein Binding; Protein Biosynthesis; Protein Processing, Post-Translational; Protein Structure, Tertiary; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-mdm2; RNA Folding; RNA Processing, Post-Transcriptional; RNA, Messenger; Sf9 Cells; Spodoptera; Substrate Specificity; Tumor Suppressor Protein p53
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