The effect of caffeic acid phenethyl ester analogues in a modified resistant hepatocyte model
Article
-
- Overview
-
- Research
-
- Identity
-
- Additional Document Info
-
- View All
-
Overview
abstract
-
We present a study of the chemoprotective effects of two caffeic acid phenethyl ester (CAPE)-related structures: LQM717 and LQM706. The modified resistant hepatocyte model in rats was used to study the chemoprevention of these CAPE analogues, which are inexpensive and easily obtained. In the liver cancer model used, we detected extensive necrosis and lipid peroxidation after 24 h, many altered hepatic foci, putatively preneoplastic lesions with γ-glutamyl transpeptidase staining after 30 days, and liver tumors at 12 months. We tested the effect of the CAPE analogues on necrosis, lipid peroxidation, proliferation, p65 activation, altered hepatic foci, and tumors. Both compounds exerted protective effects on lipid peroxidation, necrosis, cell proliferation, p65 activation, and preneoplastic lesions. Rats under a carcinogenic protocol showed a 52, 71.74, and 51.6%25 decrease in the number of preneoplastic nodules when pretreated with CAPE, LQM706, and LQM717, respectively. At 12 months after carcinogenic treatment, eight of eight rats developed liver cancer, whereas in the group of rats that received pretreatment with CAPE, LQM706, or LQM717, 62.5, 83.3, or 42.85%25, respectively, had tumors. In conclusion, LQM717 has the potential to enhance chemoprotection activity much better than CAPE by markedly reducing the formation of liver cancers in this model, and this is a compound that is easy to obtain. © 2013 Wolters Kluwer Health | Lippincott Williams %26 Wilkins.
-
We present a study of the chemoprotective effects of two caffeic acid phenethyl ester (CAPE)-related structures: LQM717 and LQM706. The modified resistant hepatocyte model in rats was used to study the chemoprevention of these CAPE analogues, which are inexpensive and easily obtained. In the liver cancer model used, we detected extensive necrosis and lipid peroxidation after 24 h, many altered hepatic foci, putatively preneoplastic lesions with γ-glutamyl transpeptidase staining after 30 days, and liver tumors at 12 months. We tested the effect of the CAPE analogues on necrosis, lipid peroxidation, proliferation, p65 activation, altered hepatic foci, and tumors. Both compounds exerted protective effects on lipid peroxidation, necrosis, cell proliferation, p65 activation, and preneoplastic lesions. Rats under a carcinogenic protocol showed a 52, 71.74, and 51.6%25 decrease in the number of preneoplastic nodules when pretreated with CAPE, LQM706, and LQM717, respectively. At 12 months after carcinogenic treatment, eight of eight rats developed liver cancer, whereas in the group of rats that received pretreatment with CAPE, LQM706, or LQM717, 62.5, 83.3, or 42.85%25, respectively, had tumors. In conclusion, LQM717 has the potential to enhance chemoprotection activity much better than CAPE by markedly reducing the formation of liver cancers in this model, and this is a compound that is easy to obtain. © 2013 Wolters Kluwer Health | Lippincott Williams & Wilkins.
publication date
published in
Research
keywords
-
caffeic acid phenethyl ester; chemical carcinogenesis; chemoprotection; hepatocellular carcinoma caffeic acid derivative; caffeic acid phenethyl ester; gamma glutamyltransferase; lqm 706; lqm 717; n (2 chlorobenzyl) 2 phenylacetamide; n (2 chlorobenzyl)cinnamamide; synaptotagmin I; unclassified drug; animal experiment; animal model; animal tissue; article; cancer cell; cell proliferation; controlled study; drug effect; lipid peroxidation; liver cancer; liver necrosis; liver nodule; male; nonhuman; priority journal; rat; staining; 2-Acetylaminofluorene; Acetanilides; Animals; Anticarcinogenic Agents; Antioxidants; Caffeic Acids; Carcinogens; Cell Division; Cinnamates; Diethylnitrosamine; Drug Evaluation, Preclinical; Drug Resistance; Glutathione S-Transferase pi; Hepatectomy; Hepatocytes; Ki-67 Antigen; Lipid Peroxidation; Liver Neoplasms, Experimental; Male; Molecular Structure; Phenylethyl Alcohol; Precancerous Conditions; Rats; Rats, Inbred F344; Transcription Factor RelA
Identity
Digital Object Identifier (DOI)
PubMed ID
Additional Document Info
start page
end page
volume
issue