abstract

• Porous silicon microcavity (PSiMc) structures were used to immobilize the photosynthetic reaction center (RC) purified from the purple bacterium Rhodobacter sphaeroides R-26. Two different binding methods were compared by specular reflectance measurements. Structural characterization of PSiMc was performed by scanning electron microscopy and atomic force microscopy. The activity of the immobilized RC was checked by measuring the visible absorption spectra of the externally added electron donor, mammalian cytochrome c. PSi/RC complex was found to oxidize the cytochrome c after every saturating Xe flash, indicating the accessibility of specific surface binding sites on the immobilized RC, for the external electron donor. This new type of bio-nanomaterial is considered as an excellent model for new generation applications of silicon-based electronics and biological redox systems. © 2012 Hajdu et al.

authors

• Agarwal V.
• Gergely C.
• Hajdu K.
• Hernádi K.
• Martin M.
• Nagy L.
• Németh Z.
• Palestino Escobedo Alma Gabriela
• Zimányi L.

publication date

• 2012-01-01

funding provided via

• Consejo Nacional de Ciencia y Tecnología, CONACYT: 122017  Grant
• Magyar Tudományos Akadémia, MTA  Grant
• SH/7/2/20, TCooperationProgram,projectno.10-1-2011-0735  Grant
• Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, SNF: IZ73Z0_128037/1  Grant

published in

• Nanoscale Research Letters  Journal

keywords

• Biophotonics; Nanomaterial; Peptide; Photosynthetic reaction center; Porous silicon functionalization Atomic force microscopy; Binding sites; Crystal atomic structure; Mammals; Nanostructured materials; Peptides; Photonics; Photosynthesis; Redox reactions; Scanning electron microscopy; Bio photonics; Functionalizations; Photosynthetic reaction center; Porous silicon microcavities; Rhodobacter sphaeroides; Silicon-based electronics; Structural characterization; Visible absorption spectra; Porous silicon; Bacteria (microorganisms); Mammalia; Proteobacteria; Rhodobacter sphaeroides

Digital Object Identifier (DOI)

• 10.1186/1556-276X-7-400

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volume

• 7