Dynamic modulation of short-term synaptic plasticity in the auditory cortex: The role of norepinephrine
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Norepinephrine (NE) is an important modulator of neuronal activity in the auditory cortex. Using patch-clamp recording and a pair pulse protocol on an auditory cortex slice preparation we recently demonstrated that NE affects cortical inhibition in a layer-specific manner, by decreasing apical but increasing basal inhibition onto layer II/III pyramidal cell dendrites. In the present study we used a similar protocol to investigate the dependence of noradrenergic modulation of inhibition on stimulus frequency, using 1s-long train pulses at 5, 10, and 20 Hz. The study was conducted using pharmacologically isolated inhibitory postsynaptic currents (IPSCs) evoked by electrical stimulation of axons either in layer I (LI-eIPSCs) or in layer II/III (LII/III-eIPSCs). We found that: 1) LI-eIPSC display less synaptic depression than LII/III-eIPSCs at all the frequencies tested, 2) in both type of synapses depression had a presynaptic component which could be altered manipulating [Ca2%2b]o, 3) NE modestly altered short-term synaptic plasticity at low or intermediate (5-10 Hz) frequencies, but selectively enhanced synaptic facilitation in LI-eIPSCs while increasing synaptic depression of LII/III-eIPSCs in the latest (>250 ms) part of the response, at high stimulation frequency (20 Hz). We speculate that these mechanisms may limit the temporal window for top-down synaptic integration as well as the duration and intensity of stimulus-evoked gamma-oscillations triggered by complex auditory stimuli during alertness. © 2010.
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calcium ion; noradrenalin; alertness; animal tissue; article; auditory cortex; auditory stimulation; brain nerve cell; brain slice; controlled study; dendrite; electrostimulation; inhibitory postsynaptic potential; nerve cell plasticity; nerve fiber; nonhuman; patch clamp; presynaptic potential; priority journal; pyramidal nerve cell; rat; synaptic transmission; Animals; Auditory Cortex; Calcium Signaling; Electric Stimulation; Evoked Potentials; gamma-Aminobutyric Acid; Inhibitory Postsynaptic Potentials; Models, Neurological; Neuronal Plasticity; Norepinephrine; Patch-Clamp Techniques; Rats; Rats, Sprague-Dawley; Synapses
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