Immunogenicity of nuclear-encoded LTB:ST fusion protein from Escherichia coli expressed in tobacco plants
Article
-
- Overview
-
- Research
-
- Identity
-
- Additional Document Info
-
- View All
-
Overview
abstract
-
Enterotoxigenic Escherichia coli (ETEC) is one of the main causative agents of diarrhea in infants and for travelers. Inclusion of a heat-stable (ST) toxin into vaccine formulations is mandatory as most ETEC strains can produce both heat-labile (LT) and ST enterotoxins. In this study, a genetic fusion gene encoding for an LTB:ST protein has been constructed and transferred into tobacco via Agrobacterium tumefaciens-mediated transformation. Transgenic tobacco plants carrying the LTB:ST gene are then subjected to GM1-ELISA revealing that the LTB:ST has assembled into pentamers and displays antigenic determinants from both LTB and ST. Protein accumulation of up to 0.05%25 total soluble protein is detected. Subsequently, mucosal and systemic humoral responses are elicited in mice orally dosed with transgenic tobacco leaves. This has suggested that the plant-derived LTB:ST is immunogenic via the oral route. These findings are critical for the development of a plant-based vaccine capable of eliciting broader protection against ETEC and targeting both LTB and ST. Features of this platform in comparison to transplastomic approaches are discussed. © 2011 Springer-Verlag.
publication date
funding provided via
published in
Research
keywords
-
Broader protective vaccine; Chimeric protein; Oral immunization antigen; bacterial toxin; enterotoxin; heat stable toxin (E coli); hybrid protein; protein subunit; amino acid sequence; animal; antibody production; article; cell nucleus; chemistry; Escherichia coli; genetics; immunology; metabolism; molecular genetics; mouse; nucleotide sequence; oral drug administration; polymerase chain reaction; tobacco; transgenic plant; Administration, Oral; Amino Acid Sequence; Animals; Antibody Formation; Antigens; Bacterial Toxins; Base Sequence; Cell Nucleus; Enterotoxins; Escherichia coli; Mice; Molecular Sequence Data; Plants, Genetically Modified; Polymerase Chain Reaction; Protein Subunits; Recombinant Fusion Proteins; Tobacco; Agrobacterium tumefaciens; Escherichia coli; Mus; Nicotiana obtusifolia; Nicotiana tabacum
Identity
Digital Object Identifier (DOI)
PubMed ID
Additional Document Info
start page
end page
volume
issue