Noninvasive detection of filaggrin gene mutations using Raman spectroscopy
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Knowledge of the existence of filaggrin (FLG) gene mutations might be helpful for a subclassification of patients with atopic dermatitis (AD) which can be used to introduce individualized treatments. In this work the filaggrin content in the skin is assessed using Raman spectroscopy and the results are compared to FLG genotyping of Mexican-mestizo patients. Results showed that the 2282del4 and R501X mutations present in the European population but absent in people of Asian or African descent are also present in the Mexican-mestizo population. The results also showed that patients with filaggrin gene mutations presented lower filaggrin concentrations measured using the vector correlation of their skin Raman spectra and a fixed spectrum of pure human recombinant filaggrin, these results indicate that Raman spectroscopy may be used as a noninvasive tool to detect FLG gene mutations. © 2011 Optical Society of America.
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Dermatitis; Raman spectroscopy; Atopic dermatitis; Fixed spectrum; Gene mutations; Genotyping; Non-invasive detection; Noninvasive tools; Skin Raman; Vector correlations; Genes
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