Pattern of expression of apoptosis and inflammatory genes in humans exposed to arsenic and/or fluoride
Article
-
- Overview
-
- Research
-
- Identity
-
- Additional Document Info
-
- View All
-
Overview
abstract
-
We have assessed whether the combined exposure to arsenic (As) and fluoride (F) exerts a different effect than the exposure to As alone on the pattern of expression of apoptosis and inflammatory genes by immune cells. RNA was extracted from peripheral blood mononuclear cells from twenty individuals exposed or not to As or F or both. Then, cDNA was isolated, and the expression of 180 genes related to apoptosis and inflammation was tested by a cDNA array test. We found significant differences in the expression of 9 apoptosis and 15 inflammation genes in the three exposed groups compared to non-exposed individuals. In addition, subjects exposed to As or F or both showed different patterns of expression of at least 19 genes. Our data indicate that the combined exposure to As and F has a different effect on gene expression than the exposure to As or F alone. © 2009 Elsevier B.V. All rights reserved.
publication date
funding provided via
published in
Research
keywords
-
Apoptosis; Arsenic; Fluoride; Gene expression; Inflammation; Macroarrays Apoptosis; cDNA arrays; Different effects; Immune cells; Inflammatory genes; Macroarrays; Peripheral blood mononuclear cells; Arsenic; Bioactivity; Fluorine containing polymers; Gene expression; Nucleic acids; Pathology; RNA; Cell death; arsenic; caspase; complementary DNA; drinking water; fluoride; inhibitor of apoptosis protein; protein bcl 2; tumor necrosis factor; tumor necrosis factor receptor; tumor necrosis factor receptor associated factor; apoptosis; arsenic; bioassay; cell organelle; DNA; fluoride; gene expression; immunity; adult; apoptosis; article; blood sampling; cell isolation; controlled study; DNA isolation; exposure; gene expression; human; human cell; immunocompetent cell; inflammation; peripheral blood mononuclear cell; priority journal; reverse transcription polymerase chain reaction; RNA extraction; Adolescent; Adult; Apoptosis; Arsenicals; Cell Survival; Fluorides; Gene Expression Profiling; Gene Expression Regulation; Humans; Inflammation; Leukocytes, Mononuclear; Middle Aged; Oligonucleotide Array Sequence Analysis; Reverse Transcriptase Polymerase Chain Reaction; RNA; RNA, Messenger; Water Supply; Young Adult
Identity
Digital Object Identifier (DOI)
PubMed ID
Additional Document Info
start page
end page
volume
issue