Anti-CD43 and anti-galectin-1 autoantibodies in patients with systemic lupus erythematosus
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Objectives: Systemic lupus erythematosus (SLE) is characterized by the production of multiple autoantibodies and also by T-cell dysfunction. CD43 is expressed by most immune cells, is involved in lymphocyte adhesion and activation, and interacts with galectin-1 (Gal-1). The aim of this work was to evaluate the plasma levels of autoantibodies against CD43 and Gal-1 as well as the levels of soluble Gal-1 in SLE Mexican mestizo patients, with the aim of establishing a correlation between these parameters and the clinical profile. Methods: Serum levels of immunoglobulin (Ig)G autoantibodies against CD43 and Gal-1 and levels of soluble Gal-1 were measured by enzyme-linked immunosorbent assay (ELISA) in 55 patients with SLE and 71 healthy controls. Results: We found significantly enhanced titres of anti-CD43 and anti-Gal-1 antibodies in sera from SLE patients compared to controls. In addition, the serum levels of Gal-1 were significantly higher in SLE patients than in healthy individuals. However, we could detect no correlation of these parameters with disease activity [using the Mexican Systemic Lupus Erythematosus Disease Activity Index (MEX-SLEDAI)], age, or a variety of different clinical or laboratory features. Similarly, no significant correlation with immunosuppressive or glucocorticoid therapy was observed. By contrast, a significant association was found between anti-CD43 titres and time of disease evolution, complement levels, and the presence of anti-Gal-1 antibodies. Conclusions: As CD43 and Gal-1 participate in modulating the immune system, we suggest that the presence of autoantibodies against these molecules may contribute to the immune deregulation observed in SLE. © 2010 Informa UK Ltd.
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autoantibody; azathioprine; CD23 antigen; chloroquine; complement; cyclophosphamide; galectin 1; glucocorticoid; immunoglobulin G antibody; methotrexate; methylprednisolone; prednisone; adult; age distribution; antibody titer; article; clinical feature; complement blood level; controlled study; deregulation; disease activity; disease association; disease course; enzyme linked immunosorbent assay; female; human; immunoglobulin blood level; immunomodulation; immunosuppressive treatment; laboratory; major clinical study; male; Mexico; molecule; priority journal; systemic lupus erythematosus; Adolescent; Adult; Age Factors; Antigens, CD43; Autoantibodies; Biological Markers; Case-Control Studies; Enzyme-Linked Immunosorbent Assay; Female; Galectin 1; Humans; Lupus Erythematosus, Systemic; Male; Prognosis; Reference Values; Risk Assessment; Severity of Illness Index; Sex Factors; Young Adult
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