Quantitation of human papillomavirus type 16 E6 oncogene sequences by real-time or quantitative PCR with EvaGreen
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Quantitation of E6 oncogene sequences of the human papillomavirus type 16 by real-time or quantitative PCR (qPCR) is used to determine the viral load, which correlates with the degree of the cervical neoplastic lesions. In the presence of EvaGreen, a new DNA intercalating fluorochrome, we obtained consistent and reproducible qPCR amplification curves and thermal denaturation profiles identical to those of the authentic E6-HPV16 (human papillomavirus 16) genome from the amplification products derived from a construct carrying the E6-HPV16 oncogene. E6-HPV16 quantitation in the presence of EvaGreen, therefore, is reproducible and specific and may be used to determine HPV16 viral load. © 2008 Elsevier Inc. All rights reserved.
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Biochemistry; Chemistry; Amplification curves; Amplification products; Fluorochromes; Human papillomavirus; Neoplastic lesions; Quantitative PCR; Thermal denaturations; Viral load; Polymerase chain reaction; evagreen; fluorochrome; article; denaturation; gene sequence; Human papillomavirus type 16; nonhuman; nucleotide sequence; oncogene; polymerase chain reaction; priority journal; quantitative polymerase chain reaction; real time polymerase chain reaction; virus detection; Human papillomavirus - 16; Human papillomavirus type 16
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