abstract
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Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) driven by the T7 promoter. The mSPpac-hINF-γ was processed and the protein was transported to periplasm. Up to 30.1%25 of hINF-γ was found in the periplasmic soluble fraction, whereas only 15%25 of the mSPpac-hIL-2 was processed, but hIL-2 was not found in the periplasmic soluble fraction. © 2007 Springer Science Business Media B.V.
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Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) driven by the T7 promoter. The mSPpac-hINF-γ was processed and the protein was transported to periplasm. Up to 30.1%25 of hINF-γ was found in the periplasmic soluble fraction, whereas only 15%25 of the mSPpac-hIL-2 was processed, but hIL-2 was not found in the periplasmic soluble fraction. © 2007 Springer Science%2bBusiness Media B.V.
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