Increased cyclosporine bioavailability induced by experimental nephrotic syndrome in rats
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Components of whole blood and plasma are highly altered during the presentation of nephrotic syndrome. The present study was aimed to explore the influence of nephrotic syndrome on the pharmacokinetics of cyclosporine (CsA) (10 mg/kg) administered i.v. to control or puromycin-induced nephrotic rats (P-NS). We found an increase in CsA bioavailability in the nephrotic group compared with controls. The area under the curve of blood CsA versus time (AUCjv) increased from 27.7 ± 5.3 to 60.6 ± 13.8 μg·h·mL-1 in control and P-NS rats, respectively. The AUCiv augmentation was positively correlated with cholesterol levels. On the other hand, the total body clearance was significantly lower (0.38 ± 0.06 vs. 0.17 ± 0.03 L·(kg body mass) -1·h-1) and the volume of distribution at steady state (3.70 ± 0.52 vs. 2.85 ± 0.32 L/kg) was significantly smaller in nephrotic rats as compared with control. These pharmacokinetic changes lead to a longer terminal half-life of CsA in P-NS rats (11.8 ± 1.6 vs. 6.9 ± 0.91 h). We conclude that the physiopathologic changes induced by the nephrotic syndrome in P-NS animals result in a significant increase in CsA blood exposure by both the decrease in drug distribution and the reduction in elimination rate of CsA. © 2007 NRC.
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Animals; Calcineurin inhibitor; Nephrotic syndrome; Pharmacokinetics; Puromycin aminonucleoside cyclosporin; cyclosporin A; puromycin; animal experiment; animal model; area under the curve; article; bioavailability; cholesterol blood level; controlled study; drug distribution; drug effect; drug half life; immunoassay; male; nephrotic syndrome; nonhuman; priority journal; protein binding; rat; single drug dose; Animals; Area Under Curve; Biological Availability; Cholesterol; Cyclosporine; Disease Models, Animal; Half-Life; Hypercholesterolemia; Hypertriglyceridemia; Hypoalbuminemia; Immunosuppressive Agents; Injections, Subcutaneous; Male; Metabolic Clearance Rate; Nephrotic Syndrome; Puromycin Aminonucleoside; Rats; Rats, Wistar
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