Renal angiotensin II concentration and interstitial infiltration of immune cells are correlated with blood pressure levels in salt-sensitive hypertension
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Renal immune cell infiltration and cells expressing angiotensin II (AII) in tubulointerstitial areas of the kidney are features of experimental models of salt-sensitive hypertension (SSHTN). A high-salt intake tends to suppress circulating AII levels, but intrarenal concentrations of AII have not been investigated in SSHTN. This study explored the relationship between these features to gain insight into the pathophysiology of SSHTN. Plasma angiotensin II (AII) and renal interstitial AII (microdialysis technique) and the infiltration of macrophages, lymphocytes, and AII-positive cells were determined in SSHTN induced by 5 wk of a high-salt diet (HSD) after short-term infusion of AII in rats with (n = 10) and without (n = 11) treatment with mycophenolate mofetil (MMF) and in control rats fed a high- (n = 7) and normal (n = 11) salt diet. As in previous studies, MMF did not affect AII-associated hypertension but reduced the interstitial inflammation and the SSHTN in the post-AII-period. During the HSD period, the AII group untreated with MMF had mean ± SD) low plasma (2.4 ± 1.4 pg/ml) and high interstitial AII concentration (1,310 ± 208 pg/ml); MMF treatment resulted in a significantly lower interstitial AII (454 ± 128 pg/ml). Renal AII concentration and the number of tubulointerstitial AII-positive cells were correlated. Blood pressure correlated positively with interstitial AII and negatively with plasma AII, thus giving compelling evidence of the paramount role of the AII within the kidney in the AII-induced model of salt-driven hypertension. Copyright © 2007 the American Physiological Society.
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Lymphocytes; Macrophages; Renal hypertension angiotensin II; mycophenolic acid 2 morpholinoethyl ester; angiotensin blood level; animal experiment; animal model; article; blood pressure; cell infiltration; controlled study; correlation analysis; hypertension; immunocompetent cell; interstitial nephritis; kidney; kidney interstitium; lymphocyte; macrophage; male; microdialysis; nonhuman; priority journal; rat; statistical significance; Angiotensin II; Animals; Anti-Inflammatory Agents, Non-Steroidal; Blood Pressure; Hypertension, Renal; Immunohistochemistry; Infusion Pumps, Implantable; Kidney; Lymphocytes; Macrophages; Male; Microdialysis; Mycophenolic Acid; Rats; Rats, Sprague-Dawley; Sodium, Dietary
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