Micromethod for the determination of cefepime by high performance liquid chromatography [Micrométodo para la determinación de cefepime en plasma mediante cromatografía de líquidos de alta resolución]

a modified analytical method was validated to quantify Cefepime in plasmatic microsamples by liquid chromatography. The analytic procedure was conducted at 4°C with 100 μL of plasma. The analytical methodology showed selectivity for cefepime, it was lineal in the range of 5 to 50 μg/mL with a correlation coefficient (r) of 0.9951, the absolute recovery was 101.89 ± 4.6%25 and its variation coefficient was 4.5%25. The accuracy was demonstrated with variation coefficients smaller than 4.44%25 and the quantifiable average value for exactitude was within 8.25%25 of the nominal value. The drug showed stability for 30 days at -80°C. This method comply with the validation parameters established by the Norma Oficial Mexicana (NOM-177-SSA1-1998) to be applied in pharmacokinetics studies of this cephalosporin in neonates.

Cefepime; HPLC; Newborn cefepime; accuracy; analytic method; article; correlation coefficient; drug blood level; drug determination; high performance liquid chromatography; human; low temperature; newborn; sample size

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