Apical Periodontitis Healing Following Treatment is Impacted by Root Canal Sealer Composition: An in Vivo and in Vitro Investigation
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Objective: To evaluate the periapical healing following root canal treatment in teeth with apical periodontitis (in vivo) and the cytotoxic potential of root canal sealers in vitro. Material and Methods: Apical periodontitis was induced in 60 dogs%27 teeth and root canals were filled with Sealapex (40 roots), EndoREZ (40 roots), intracanal dressing (20 roots), or left untreated (20 roots). After 30 and 90 days, histopathological analyses were made. In vitro, J774.1 macrophages were stimulated with root canal sealers extracts, cytotoxicity was assessed using lactate dehydrogenase assay, and qRT-PCR was used to analyze TNF-α gene expression. Results: In vivo, smaller apical periodontitis and lower inflammatory cell infiltrate were found in teeth treated with Sealapex compared to EndoREZ. In vitro, EndoREZ was cytotoxic and induced TNF-α gene expression by macrophages differently from Sealapex. Conclusion: Sealapex allowed improved tissue repair following root canal treatment in teeth with apical periodontitis compared to EndoREZ. Synthesis of TNF-α induced by LPS was enhanced by EndoREZ, whereas Sealapex prevented pro-inflammatory gene expression. © 2022, Association of Support to Oral Health Research (APESB). All rights reserved.
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Endodontics; Periapical Diseases; Root Canal Obturation isoflurane; lactate dehydrogenase; lactic acid; levomepromazine; lipopolysaccharide; noradrenalin; sodium chloride; tiletamine plus zolazepam; tumor necrosis factor; animal experiment; animal model; animal tissue; Article; cell infiltration; controlled study; cytotoxicity assay; endodontics; histology; in vitro study; in vivo study; macrophage; MTT assay; nonhuman; osteoblast; osteolysis; polymerase chain reaction; protein expression; real time polymerase chain reaction; RNA isolation; scanning electron microscopy; tissue repair; tooth periapical disease; tooth root canal
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