Amarisolide H and 15-epi-Amarisolide H, Two Diterpenoid Glucosides from Salvia circinnata
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The HPLC–PDA profiling of an acetone-soluble extract of the leaves of Salvia circinnata Cav. (Syn. Salvia amarissima Ortega), Lamiaceae, collected at Patzcuaro, Michoacan, Mexico, indicated this population corresponds to the chemotype “amarisolide A.” The phytochemical study of the extract led to the isolation of two new diterpenoid glucosides, amarisolide H and 15-epi-amarisolide H, which were obtained as an epimeric mixture, together with the known compounds amarisolides A, D, G, 16-epi-amarisolide G, and 5,6-dihydroxy-7,3′,4′-trimethoxyflavone. The structures of compounds amarisolide H and 15-epi-amarisolide H were determined by the analyses of their NMR and HRMS data. The cytotoxicity and anti-multidrug resistance (MDR) effects of compounds amarisolide H, 15-epi-amarisolide H, amarisolide A, amarisolide G, and 16-epi-amarisolide G in MCF-7 cancer cells sensible and resistant to vinblastine were essayed. Graphical Abstract: [Figure not available: see fulltext.] © 2022, The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia.
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Breast cancer; Cytotoxicity; Mexican flora; Mint family; Structure elucidation; Terpenoids 15 epi amarisolide h; 16 epi amarisolide g; Amarisolide; amarisolide a; amarisolide g; amarisolide h; carbohydrate derivative; diterpenoid; glucoside; plant extract; reserpine; Salvia circinnata extract; unclassified drug; vinblastine; Article; breast cancer; Caov-3 cell line; carbon nuclear magnetic resonance; controlled study; cytotoxicity; HCT 116 cell line; HeLa cell line; high performance liquid chromatography; human; human cell; IC50; Lamiaceae; MCF-7 cell line; multidrug resistance; nuclear magnetic resonance; optical rotation; proton nuclear magnetic resonance; Salvia circinnata; thin layer chromatography
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