abstract

• A composite containing hydroxyapatite, graphene oxide and silver nanoparticles was obtained by performing a microwave-assisted hydrothermal method, a modified Hummer\%27s synthesis, and advantageously, by using dietary quercetin for silver nanoparticles formation and deposition. The pure constituents and composites were char-acterized by complementary physicochemical techniques, and evaluated in human keratinocytes and bacteria. In eukaryotic cells, it was found a dose-dependent toxic response that mainly depends on the presence of graphene oxide, and a superior cytocompatibility was observed for the samples containing hydroxyapatite. The ternary system showed a half maximal inhibitory concentration of about 116.8 & mu;g/mL, ranged in a safety threshold. In comparison with silver nanoparticles alone, this ternary composite increased in 43%25 and 38%25 the inhibition zone for S. aureus and E. coli, respectively. Its minimum inhibitory concentration was found, respectively, close to 6.5 and 52.1 & mu;g/mL, resulting in a fractional inhibitory concentration index < 0.5. Thus, this synergistic system can potentially be applied as a bactericidal for tissue replacement and surface coating.

authors

• González Calderón José Amir
• Pérez López José Elías
• Rodríguez-Lugo V.
• Salado-Leza D.

publication date

• 2023-01-01

funding provided via

• Consejo Nacional de Ciencia y Tecnologia (CONACYT) [102986]; CONACYT [299818, 302286, A1-S-45958]; CONACYT  Grant

published in

• Materials Today Communications  Journal

keywords

• Hydroxyapatite; Graphene oxide; Silver nanoparticles; Cytotoxicity; Antibacterial activity

Digital Object Identifier (DOI)

• 10.1016/j.mtcomm.2023.106097

PubMed ID

start page

end page

volume

• 35

issue